Defective populations of regulatory T cells correlate with the occurrence of chronic graft versus host disease after allogeneic stem cell transplantation

E. Zorn, B. Floyd, S.J. Lee, R.J. Soiffer, J.H. Antin, J. Ritz
2004 Biology of Blood and Marrow Transplantation  
be insufficient to identify Treg in humans. To determine whether the ICOSϩ T-cell populations which were modulated with ECP treatment were functionally Treg, we isolated CD4ϩCD25ϩ ICOSϩ and CD4ϩCD25ϩ ICOS-cells from patients with cGVHD before and after ECP therapy and demonstrated that the CD4ϩCD25ϩICOSϩ cells functioned as suppressors in an MLR reaction, suggesting that these cells represent Treg cells. We conclude that these ICOS ϩ CD25 ϩ CD4 ϩ T-helper cells may represent, in part,
more » ... latory T-cells implicated in the induction of tolerance. We propose that the mechanism of action of ECP in cGVHD involves both modulation of DC to induce maturation markers, including possibly the ligand for ICOS (B7H2), as well as the induction of ICOS expressing CD25 ϩ immunoregulatory T-helper cells.
doi:10.1016/j.bbmt.2003.12.074 fatcat:rputrm5x3nhddh6n2o2zyoie3u