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be insufficient to identify Treg in humans. To determine whether the ICOSϩ T-cell populations which were modulated with ECP treatment were functionally Treg, we isolated CD4ϩCD25ϩ ICOSϩ and CD4ϩCD25ϩ ICOS-cells from patients with cGVHD before and after ECP therapy and demonstrated that the CD4ϩCD25ϩICOSϩ cells functioned as suppressors in an MLR reaction, suggesting that these cells represent Treg cells. We conclude that these ICOS ϩ CD25 ϩ CD4 ϩ T-helper cells may represent, in part,doi:10.1016/j.bbmt.2003.12.074 fatcat:rputrm5x3nhddh6n2o2zyoie3u