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run at 4°. Packed columns were washed with 2 M NaCl solution until no further material absorbing at 260 m@.t was eluted and then equilibrated with 0.4 M NaCl solution, 0.01 M MgCl2 , and 0.05 M sodium acetate, pH 5 .0. All subse quent solutions were made up in sodium acetate buffer, pH 5.0, and 0.01 M MgCl2 . Samples of tRNA were loaded onto the column in 0.4 M NaCl solution. Unacylated tRNA was eluted with a linear gradient of 500 ml of 0.4 M NaC1 solution and 500 ml of 1.0 M NaCl solution.pmid:5528921 fatcat:6pzi2bikxvg23j3bjpczht4bgi