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1 Extraction of high quality DNA for long read sequencing e.g. PacBio Optimized for DNA extraction from wheat stripe rust spores and also tested on barley leaf rust. Buffers are best when fresh and not older than 3-6 months. Buffered Phenol:Chloroform:Isoamylalcohol (25:24:1) should not be older than 3 months. Critical steps to obtain high quality DNA: Do NOT heat samples during DNA extractions! Perform all steps at RT or 4oC as indicated. Do NOT incubate samples with KAc for prolonged timedoi:10.17504/protocols.io.ewdbfa6 fatcat:5rurkkiuzvbdjbxnetcpaw2lqe