Inhibitory Kinetics of 2'-Hydroxy-4'-methoxyacetophenone on Tyrosinase-catalyzing Reaction

Shengzhao Gong, Qingsheng Chen, Mengyi Xu
2016 Proceedings of the 2015 4th International Conference on Sensors, Measurement and Intelligent Materials   unpublished
Abstract:The inhibitory effects of 2'-hydroxy-4'-methoxyacetophenone on the activity diphenolase contained in tyrosinase was studied using enzymological kinetic method. 2'-hydroxy-4'-methoxyacetophenone was found to inhibit diphenolase activity of tyrosinase. The 2'-hydroxy-4'-methoxyacetophenone concentration leading to 50 % activity lost (IC50) was 0.60 mmol/L for diphenolase activity. The inhibition kinetics analyzed by Lineweaver-Burk plots found 2'-hydroxy-4'-methoxyacetophenone to be a
more » ... ophenone to be a mixed inhibitor for the oxidation of L-DOPA, the equilibrium constants for inhibitor binding with free enzyme (E), KI, and with enzyme-substract (ES) complex, KIS, were 0.08mmol·L-1 and 0.12 mmol·L-1 respectively.
doi:10.2991/icsmim-15.2016.117 fatcat:z5tsdlrazzeo5dn6y2k7vpcvwq