This paper deals with the cytotoxic chaetoglobosins obtained from culture on maize of the strains ATCC 26115 and MRC 2654 of the fungusPhomopsis leptostromiformisBoth strains produced, in more or less important amounts, the known chaetoglobosins M (1) and N (2). In addition, the new chaetoglobosin O (3), was isolated from the ATCC 26115 strain and characterized by HRMS and 2D NMR experiments. With the MRC 2654 strain, a fungal metabolite4, more polar than the chaetoglobosins M and N, was

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... ed and purified. Spectroscopic and chromatographic data of metabolite4led to its identification to the bridged chaetoglobosin named compoundS, which previously has been obtained by intramolecular Michael reaction on the chaetoglobosin M. Moreover, a methylation under Williamson conditions of the chaetoglobosin4resulted in the formation of the trimethylated chaetoglobosin5.