Microalgae are potential producers of lipids that are possibly used as an alternative raw material in biodiesel and drug production. This study, therefore, obtained lipids from the microalgae Nocochloropsis oculata (N. oculata), which was isolated from West Sumatera, and tests were conducted for the antimicrobial and anti-fungal properties of fatty acids and fatty acid methyl esters (FAME) present. Also, the extraction process involved ultrasonication with n-hexane solvent at a ratio of 1: 8 (1

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... mg dry N.oculata extracted with 8 mL n-hexane), and transesterification was performed subsequently with methanol, using H 2 SO 4 as a catalyst to form fatty acids and FAME, which were evaluated using disc diffusion. Meanwhile, the antimicrobial activity against Staphylococcus aureus (a grampositive bacteria), Escherichia coli (a gram-negative bacteria), and Candida albicans (a fungus) were recorded, subsequently, the fatty acid and FAME concentration used were 100, 200, and 300 mg/L. The results show a relatively higher antibacterial activity in N.oculatafatty acids than FAMEs and no antifungal effect against Candida albicans. Also, gas chromatography-mass spectrometry identified the lipid to mainly consist of palmitic acid, stearic acid, methyl laurate, and methyl stearate.