In vitro Cultivation of Hancornia speciosa Gomes : The Physical Constitution of the Culture Medium, Sucrose Concentrations and Growth Conditions

Juliana Silva Rodrigues Cabral, Paula Sperotto Alberto, Flávia Dionísio Pereira, Edson Luiz Souchie, Fabiano Guimarães Silva
2014 Plant Tissue Culture and Biotechnology  
The effect of constitution of the culture medium, different concentrations of sucrose, and different growth conditions on the germination of seeds and growth of explants of ʺmangabaʺ (Hancornia speciosa Gomes) were evaluated in two in vitro assays. In assay one, the physical constitution of a medium (solid and liquid) and different concentrations of sucrose (0, 15, 30, 45, 60, 75, and 90 g/l) were tested, and seed germination was found to be variable. In assay two evaluation of the effect of
more » ... ferent growth conditions (with and without agitation) on the in vitro growth of explants. Present results showed that ʺmangabaʺ had the highest percentage of germination and potentiality when inoculated in a liquid medium with (15 g/l) or without sucrose. The medium without agitation resulted in a better growth of explants the average length number of buds.
doi:10.3329/ptcb.v23i2.17519 fatcat:rzzbfak5fnfszph5jfwpejtxsy