Acute phase proteins: a potential approach for diagnosing chronic infection by Trypanosoma vivax

Katyane de Sousa Almeida, Alinny Ferreira Costa, Paulo Cesar da Silva, José Jurandir Fagliari, Rosangela Zacarias Machado, Adjair Antonio do Nascimento
2012 Revista Brasileira de Parasitologia Veterinária  
The present study aimed to assess potential changes in acute phase proteins in sheep experimentally infected with Trypanosoma vivax. There were studied eight male sheep, four used as controls and four infected with 10(5) T. vivax trypomastigotes. Blood samples were collected at two points times before infection and then at 5,7, 9, 11, 13, 15, 20, 30, 45, 60, 75, 90, 105 and 120 days post-infection (dpi). Blood samples were centrifuged and allotted, and acute phase proteins were then separated
more » ... electrophoresis on acrylamide gel containing sodium dodecyl sulfate. Protein concentrations were determined by computer-assisted densitometry. Total protein was determined by colorimetric biuret method. Trypanosomes were counted daily using a 5 mL aliquot of blood smear on a glass slide under a 22 × 22 mm coverslip. Parasites were counted in 100 microscopic fields (40× magnification), and then multiplied by a correction factor. The results were expressed as parasites per mL of blood. For statistical analyses, we used the Wilcoxon test at 5% significance level. There was found a reduction in several acute phase proteins and increase in antitrypsin and transferrin. This finding can be used for the diagnosis of T. vivax infection, especially in chronic infection.
doi:10.1590/s1984-29612012000200005 pmid:22832747 fatcat:ntixl52hprc7zftozqlgndazey