Objective: The objective of the present study was to analyze the antibacterial activity of seeds of wild and hybrid varieties of Syzygium cumini against plant pathogens such as Erwinia herbicola, Pseudomonas syringae, and Xanthomonas campestris. Methods: The phytochemicals from the wild and hybrid varieties of S. cumini seeds collected from the Tirunelveli region (Tamil Nadu) were extracted by cold methanol method. Antibacterial activity of the methanol extract of seeds has been tested against

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... . herbicola, P. syringae, and X. campestris by disc diffusion assay. Results: The seed extract of wild variety revealed more effective antimicrobial activity than a hybrid variety with minimum inhibitory concentration ranging from 8 μg/ml to 128 μg/ml. The highest inhibition zone was exhibited by wild seed extract against X. campestris (16.3 mm). Conclusions: This result showed that the wild variety of S. cumini seeds may be the best antibacterial agent against plant pathogens.