Influenza virus can cause acute respiratory infection, which affects from 5% to 20% of the human population every year. Influenza virus epidemic spreads in a short time due to the high-level contagiousness and a wide range of hosts. In addition, several pandemic strains with high virulence appeared in the past, and constantly there is a threat of a new pandemic strain. The impact of influenza virus on human cells is directly related to a change in the RNA profile. Currently, scientists are

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... asingly paying attention to the study of miRNAs that are able to regulate gene expression. The study of the quantitative and qualitative composition of microRNAs is an important tool for the diagnosis and treatment of various diseases. The research is aimed at studying the profile of miRNA, in order to understand the mechanisms of intracellular miRNAs involvement in the infection with the influenza virus of human lung epithelial cells. The work also describes the effect of RNase Bacillus pumilus on epithelial cells of human lungs of the A549 line. The aim of the work is to analyze the microRNA profile to study the effect of influenza A virus (H1N1) on epithelial cells of human lung adenocarcinoma. Total intracellular RNA was isolated using the TRIzol Reagent, including microRNA. The miRNA fraction was analyzed using next-generation sequencing on the SOLiD 550xl wildfire platform and modern bioinformatics programs. In this work 129 mature microRNAs from uninfected cells treated with Bacillus pumilus RNase and cells infected with influenza A virus (H1N1) were studied. It was found that in uninfected cells treated with RNase, there are 2 times more different miRNAs responsible for the suppression of carcinogenesis. In cells that are infected with the influenza virus, the number of miRNAs involved in the development of lung cancer and breast cancer increases. The highest expression is observed in cells infected with the influenza virus for miR-6884-5p, but the role of this miRNA in infection of the influenza virus has not yet [...]