In Vitro and In Vivo Effects of Deoxyribonucleic Acid–Based Coatings Funtionalized with Vascular Endothelial Growth Factor

Jeroen J.J.P. van den Beucken, X. Frank Walboomers, Suzan T.M. Nillesen, Matthijn R.J. Vos, Nico A.J.M. Sommerdijk, Toin H. van Kuppevelt, Roeland J.M. Nolte, John A. Jansen
2007 Tissue engineering  
Vascularization is important in wound healing and essential for tissue ingrowth into porous tissueengineering matrices. Furthermore, peri-implant tissue vascularization is known to be important for the functionality of subcutaneously implanted biosensors (e.g., glucose sensors). As a first exploration of the use of deoxyribonucleic acid (DNA)-based coatings for the optimization of biosensor functionality, this study focused on the effect of DNA-based coatings functionalized with vascular
more » ... ith vascular endothelial growth factor (VEGF) on in vitro endothelial cell behavior and vascularization of the peri-implant tissue in vivo. To that end, DNAbased coatings consisting of poly-D-lysine and DNA were functionalized with different amounts of VEGF (25 and 250 ng) and compared to non-coated controls and non-functionalized DNA-based coatings. The results demonstrated the superiority of VEGF-functionalized DNA-based coatings in increasing endothelial cell proliferation and migration in vitro over non-coated controls and non-functionalized DNA-based coatings. In vivo, a significant increase in vascularization of the peri-implant area was observed for VEGFfunctionalized DNA-based coatings. Because no dosage-dependent effects were observed, future experiments should focus on optimizing VEGF concentration for this purpose. Additionally, the administration of VEGF in combination with other (pro-angiogenic) factors should be considered.
doi:10.1089/ten.2006.0303 pmid:17373893 fatcat:zhkwl6dd2zdsldyfapsdvnvtoq