Immunocytochemical Studies of Corticotropin Releasing Factor-like Immunoreactive Innervation of Atrial Natriuretic Peptide Neurons in the Rat Diencephalon

Hiroaki SANO, Takaki HAYASHI, Seiji SHIODA, Yasumitsu NAKAI
1992 The Showa University Journal of Medical Sciences  
Synaptic inputs of corticotropin-releasing factor (CRF)-like Immuno reactive axon terminals to neurons containing atrial natriuretic peptide (ANP) in the anteroventral third ventricle (AV3V) region of the rat diencephalon were examined ultrastructurally by a pre-embedding double immunostaining technique combining the silver-gold intensified peroxidase-antiperoxidase complex-3,3'diaminobenzidine (SGI-PAP-DAB) method for ANP with the nonintensified PAP-DAB method for CRF. CRF-like Immunoreactive
more » ... xon terminals were found to make asymmetrical or symmetrical synaptic contacts with ANP-like Immunoreactive nerve cell bodies and processes in the AV3V region. These findings suggest that CRF-containing neurons may regulate the secretory activity, or other functions, or both, of ANP-containing neurons through synapses in the AV3V region of the rat diencephalon.
doi:10.15369/sujms1989.4.161 fatcat:qw3qrfhfg5dfjjcp7hciqk34ju