Single Molecule Light Field Microscopy [article]

Ruth R Sims, Sohaib Abdul Rehman, Martin O Lenz, Adam Clark, Edward W Sanders, Aleks Ponjavic, Leila Muresan, Steven F Lee, Kevin O'Holleran
2020 bioRxiv   pre-print
We introduce single molecule light field microscopy (SMLFM), a novel 3D single molecule localization technique that is capable of up to 20 nm isotropic precision across a 6 μm depth of field. SMLFM can be readily implemented by installing a refractive microlens array into the conjugate back focal plane of any widefield single molecule localization system. We demonstrate that 3D localization can be performed by post-processing 2D localization data generated by common, widely-used, algorithms. In
more » ... this work we benchmark the performance of SMLFM and finally showcase its capabilities by imaging fluorescently labeled membranes of fixed eukaryotic cells below the diffraction limit.
doi:10.1101/2020.05.20.104802 fatcat:xe4xfl3x45chbhulbo5lvnfute