groups C, PPP, PRP-sol, and PRP-sr, respectively; p ! 0.05), and mature vessel density (28 8 2; 31 8 3; 52 8 10, and 85 8 13 for the C, PPP, PRP-sol, and PRP-sr groups, respectively; p ! 0.05). Conclusion: A sustained release of PRP containing potent angiogenic growth factors restores blood perfusion by stimulating angiogenesis and arteriogenesis. Abstract Objective: The objective of this investigation was to establish the effectiveness of sustained-release platelet-rich plasma (PRP) on

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... n and neovascularization in diabetic murine hind limb ischemia. Methods: After surgery in streptozotocin-induced diabetic mice, the mice were randomly assigned to the following 4 experimental groups: control (C), 100 l of the sustained-release form of platelet-poor plasma (PPP), 100 l of the solution form of PRP (PRP-sol), and 100 l of the sustained-release form of PRP (PRP-sr). Endpoint evaluations were: blood perfusion by laser Doppler perfusion imaging (LDPI), vascular density by anti-vWF, and mature vessel density by anti-smooth muscle actin antibody. Results: This study demonstrated that a sustained release of PRP increases the perfusion of ischemic tissue as measured by LDPI (57 8 12; 56 8 9; 72 8 7, and 98 8 4 for the C, PPP, PRP-sol, and PRP-sr groups, respectively; p ! 0.05), capillary density (151 8 16; 158 8 12; 189 8 39, and 276 8 39 for