Vital Microskopic Observation of Fluorescent Labelled Platelets

G. WeBelmarm, H. Kortenhaus, H. Schröer
1979 VIIth International Congress on Thrombosis and Haemostasis   unpublished
Platelets from platelet rich plasma (PRP) of Wistar rats were labelled by incubation with fluoroisothiocyanate (FITC) in Ringer-citrate-dextrose solution. Labelled platelets were reinjected into a tail vein of a rat previously prepared for vital-microscopic observation of the mesenteric vasculature. Using a low-level-light camera, labelled platelets could be observed by a fluorescence technique as distinct luminescent particles moving with the blood stream, and their rheological behaviour could
more » ... cal behaviour could be observed. Results: Platelets moved with different velocities in the same vessel. Single platelets stuck temporarily to the vessel wall but no aggregation was observed. Extensive sticking has been observed in places of leukocyte-rolling. Using the described method it should be possible to alter platelets in vitro and to observe their behaviour in vivo after reinjection.
doi:10.1055/s-0039-1684828 fatcat:iiffz5u3srer7ekmgaexbyerpu