Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10

L V Wray, W S Reznikoff
1983 Journal of Bacteriology  
The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo repressor titration assay. The results indicate that repressor specifically binds both regions of dyad symmetry.
doi:10.1128/jb.156.3.1188-1191.1983 fatcat:5mkpkbcy2bczppokfos355hslu