Activation Mechanism of the β2-Adrenergic Receptor

Ron O. Dror, Daniel H. Arlow, Paul Maragakis, Thomas J. Mildorf, Albert C. Pan, Huafeng Xu, David W. Borhani, David E. Shaw
2012 Biophysical Journal  
lipid bilayer and control the actin-actin or actin-lipid interactions by the addition of cross-linking proteins. Upon the addition of myosin II motors, we observe the movement of actin and myosin by timelapse confocal microscopy. In the absence of adhesion to the membrane and actin cross-linking proteins, contractility at 10-100 um length scales is only observed for sufficiently long (10 um) actin filaments. To facilitate contraction of short filaments (~1 um), the addition of an actin
more » ... ker protein is required. Increasing adhesion to the lipid bilayer reduces the rate of contraction while facilitating tension build up. Our results demonstrate the roles of actin network connectivity and membrane adhesion in modulating the nature of force transmission in a biomimetic model of the actin cortex.
doi:10.1016/j.bpj.2011.11.1317 fatcat:wwba2qcajjhq7bn2talsywrkoi