To determine the HPV infection prevalence among Congolese women with normal cervical cytology in southwestern Congo, in order to establish a data baseline necessary to improve the HPV infections prevention and screening. Between November 2010 and February 2011, cervical samples of 219 women attending the General Hospital of Loandjili who had a normal cervical smear dated less than one year in cytopathological Unit register were investigated. Liquid based cytology samples were obtained using a

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... tobrush. A portion of the sample was used to confirm the cytological diagnosis and another one for the HPV detection by nested-PCR and direct sequencing. Overall, 204 women were included. HPV prevalence was 23.5% (48). HR-HPV and LR-HPV infection was detected in 29 (60.4%) and 19 (39.6%) of study participants respectively. Thirteen HPV genotypes were identified. The most common genotypes were HPV16, 13 (27%), HPV70, 8 (16.6%), HPV33 and HPV6, 5 (10.4%). A decreasing trend of HPV infection was observed as the age increases, 38.6% below 30 years to 11.1% over 60 years (p=0,379). Conclusion: HPV prevalence among Congolese study women with normal cervical cytology was high. Knowledge of the type-specific HPV distribution in women with normal cytology could help to establish the frequency in the cytological monitoring of women between two screenings.