Lane tracking software for four-color fluorescence-based electrophoretic gel images

M L Cooper, D R Maffitt, J D Parsons, L Hillier, D J States
1996 Genome Research  
Software to track sample lanes automatically in four-color, fluorescence-based, electrophoretic gel images has been developed for application in large-scale DNA sequencing projects. Lanes and lane boundaries are tracked by analyzing a first difference approximation to the gradient of a vertically integrated and processed "brightness" profile. Initially lanes are located in a region of the gel image selected for good horizontal lane spacing and signal strength. The software uses models of
more » ... es models of expected lane and interlane spacing and lateral lane behavior to maintain accurate tracking on imperfect gels. In areas where intensity-based tracking is difficult, interpixel column correlation is also used to locate and define lane features. Summary statistics and compressed-in-time images are generated for user evaluation of tracking performance. The software developed has been tested successfully on gel images with degradations including significant horizontal lane motion (curving} and image artifacts, and is now in full-scale use in our sequencing projects.
doi:10.1101/gr.6.11.1110 pmid:8938435 fatcat:wutshwyxfzdyxbg7xsm5c23cuu