Iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria

E Fautz, G Rosenfelder, L Grotjahn
1979 Journal of Bacteriology  
The fatty acids present in the total hydrolysates of several gliding bacteria (Myxococcus fulvus, Stigmatella aurantiaca, Cytophaga johnsonae, Cytophaga sp. strain samoa and Flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. In addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. The majority was odd-numbered and
more » ... nched. Small amounts of even-numbered and unbranched fatty acids were also present. Whereas 2-hydroxy-15-methylhexadecanoic acid was characteristic for myxobacteria, 2-hydroxy-13-methyltetradecanoic acid, 3-hydroxy-13-methyl-tetradecanoic acid, and 3-hydroxy-15methyl-hexadecanoic acid were dominant in the Cytophaga-Flexibacter group. heating the sample with boron trifluoride-methanol reagent (14% wt/vol Serva, Heidelberg) (16). Modification of fatty acid methyl esters. Hydrogenation of unsaturated methyl esters was performed by using nascent hydrogen (prepared with Zn-4 N HCI) with the aid of Pd on charcoal (10%; E. Merck AG, Darmstadt, Germany) as a catalyst. The 852 on May 9, 2020 by guest
doi:10.1128/jb.140.3.852-858.1979 fatcat:pn3z7tp6zve7xdefzxppmoxbka