BACKGROUND AND OBJECTIVE: Staphylococcus epidermidis is one of the most common coagulase negative Staphylococcal strains and has an important role in the infection related to medical device in human and is a crucial public in the antibiotic therapy. The aim of this study was to identify genes erythromycin resistant Staphylococcus epidermidis is isolated from children with Multiplex-PCR method. METHODS: In the cross-sectional study, a total of 60 Staphylococcus epidermidis were collected from

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... Amir-Kabir Hospital in Arak. Antibiotic susceptibility test was performed on the Muller Hinton agar according to the clinical and laboratory standard institute (CLSI). Then all strains were evaluated for ermA and ermC genes by multiplex-PCR method. FINDINGS: In the present study, the highest and lowest samples were related to urinary catheter (31 strains, 51.6%) and wound samples (11 isolates, 18.3%). All isolates were susceptible to vancomycin. The prevalence of ermA and ermC genes were 4 (6.6%) and 45 (75%), respectively. the results showed that the highest and lowest strains carried these genes were ermC and ermA, respectively. CONCLUSION: Control of transmission of the microorganisms are important infection control and classification methods phenotypic and genotypic diagnosis of clonality of isolates and better control they will be very beneficial.