Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

Ernesto Renato Krüger, Tânia Regina Penha, Fernanda Cristina Hummelgen, Jorge Bacila Agottani, Demétrio Reva, Robson Gonçalves, Vanete Thomaz-Soccol
2015 Brazilian Archives of Biology and Technology  
The aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is
more » ... ent in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
doi:10.1590/s1516-89132015050187 fatcat:h4p2qoxai5hqxga2pypuoe6eaa