Antibiotic resistance in bacteria has become a worrying phenomenon in today's world, K. pneumoniae is a member of the Enterobacteriaceae family, which causes nosocomial infections as an opportunistic pathogen but inherently harbored as a part of the natural human microbiota this study aims to inform the sensitivity of bacteria to different antibiotics commonly used in the treatment against K. pneumoniae strains isolated from different infection sites and hospital environmental samples. Samples

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... ere collected from different sources (n = 886) were recorded from Diwaniyah hospitals during the period from August 2019 to January 2020, Among the 275 isolates positive for bacterial culture, only 70 isolates (40%) were identified as K.pneumoniae by standard microbiological techniques and antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion testing to 29 antibiotics using the tablet diffusion method according to CLSI 2020, The results susceptibility testing showed that frequency resistance of K. pneumoniae isolates to Pipercillin and Amoxicillin/ clavulanic acid was (100 %), Cefuroxime )97.14%) Cefoxitin (50%), Cefotaxime (74.28%), ceftriaxone (84.28%), aztreonam (52.85%), ceftazidime (81.42%), Tetracycline (70%), Nitrofurantoin (61.42%), Amikacin (57.14%), ciprofloxacin (50%). Also, the results showed the highest frequency of susceptibility among K.pneumoniae was associated withOfloxacin(78.57%), Netilmicin(74.28%), Gentamicin(75.71%), Meropenem(68.57%), Trimethoprim-Sulfamethoxazole(64.28%), Levofloxacin(72.85%).