Neonatal septicaemia: Its bacteriological profile and antibiogram

Santosh Kotgire, Sunil Hatkar, Assitant Professor
International Journal of Medical Microbiology and Tropical Diseases   unpublished
and Objectives: Neonatal septicaemia is a life threatening clinical emergency that demands urgent diagnosis and treatment. A wide variety of bacteria can cause neonatal septicaemia. As neonatal septicaemia is a life-threatening clinical emergency, the knowledge of epidemiological and antimicrobial susceptibility pattern of common pathogens in a given area helps to inform the choice of antibiotics, therefore a regular surveillance of important bacterial isolates and their susceptibility pattern
more » ... s mandatory. So the present study was undertaken to identify the common bacterial pathogens associated with neonatal sepsis and their antibiotic susceptibility pattern. Material and Methods: The study was carried out in the Department of Microbiology, a tertiary care hospital Jalgaon, Maharashtra during the period from December 2015 to May 2016. A total 200 clinical suspected cases of neonatal septicemia were included in the study. Blood samples were collected with all aseptic precautions and inoculated on brain heart infusion broth (BHIB).Subculture were made on blood agar and Mac-conkey agar plates. Organisms were identified as per standard recommended procedures and antibiotic susceptibility testing was carried out on isolates as per Clinical Laboratory Standard Institute (CLSI) guidelines. Result: 60 different types of microrganisms were isolated. The incidence of neonatal septicemia was 30%.The prevalence of gram negative bacilli were 60.00% and gram positive bacilli were 40.00%.The commonest pathogen isolated was Klebsiella pneumonia 50.00%, followed by Staphylococcus aureus 30.00%. Klebsiella pneumonia was resistant to amikacin (86.66%) &100% resistant to ampicillin, and was 100% sensitive to pipercillin tazobactam and co-trimaxozole. The most effective antibiotics against the isolated Staphylococcus aureus were Vancomycin (100%), Linzeloid(100%), Ciprofloxacin (66%) ,Co-trimoxazole (66%) and Clindamycin (55%). Conclusion: The present study reveals microbiological profile and antibiogram of neonatal septicemia. Considering the burden of mortality resulting from septicemia, better diagnostic facilities should be employed for early detection of septicemia and rational use of narrow spectrum antibiotics is recommended.