Surfactant Proteins A and D Bind CD14 by Different Mechanisms

Hitomi Sano, Hirofumi Chiba, Daisuke Iwaki, Hitoshi Sohma, Dennis R. Voelker, Yoshio Kuroki
2000 Journal of Biological Chemistry  
Surfactant proteins A (SP- A) and D (SP-D) are lung collectins that are constituents of the innate immune system of the lung. Recent evidence (Sano, H., Sohma, H., Muta, T., Nomura, S., Voelker, D. R., and Kuroki, Y. (1999) J. Immunol. 163, 387-395) demonstrates that SP-A modulates lipopolysaccharide (LPS)-induced cellular responses by direct interaction with CD14. In this report we examined the structural elements of the lung collectins involved in CD14 recognition and the consequences for
more » ... onsequences for CD14/ LPS interaction. Rat SP-A and SP-D bound CD14 in a concentration-dependent manner. Mannose and EDTA inhibited SP-D binding to CD14 but did not decrease SP-A binding. The SP-A binding to CD14 was completely blocked by a monoclonal antibody that binds to the SP-A neck domain but only partially blocked by an antibody that binds to the SP-A lectin domain. SP-A but not SP-D bound to deglycosylated CD14. SP-D decreased CD14 binding to both smooth and rough LPS, whereas SP-A enhanced CD14 binding to rough LPS and inhibited binding to smooth LPS. SP-A also altered the migration profile of LPS on a sucrose density gradient in the presence of CD14. From these results, we conclude that 1) lung collectins bind CD14, 2) the SP-A neck domain and SP-D lectin domain participate in CD14 binding, 3) SP-A recognizes a peptide component and SP-D recognizes a carbohydrate moiety of CD14, and 4) lung collectins alter LPS/CD14 interactions. Pulmonary surfactant is a complex mixture of lipids and proteins that functions to keep alveoli from collapsing at the end of expiration (1). Surfactant proteins A (SP-A) 1 and D (SP-D) are glycoprotein constituents of lung surfactant (2). SP-A and SP-D belong to the collectin subgroup of the C-type lectin superfamily along with mannose-binding proteins A and C, conglutinin, and CL43 (3). These proteins possess similar characteristic structures consisting of a short intersubunit disulfide forming the NH 2 terminus region, a collagen-like do-
doi:10.1074/jbc.m001107200 pmid:10801802 fatcat:3q3dq5q5crezrfecdw7ymo7vl4