High-Performance Liquid Chromatography--Electrospray Ionization-Mass Spectrometry Study of Ginkgolic Acid in the leaves and Fruits of the Ginkgo Tree (Ginkgo biloba)

X.-g. He, M. W. Bernart, G. S. Nolan, L.-z. Lin, M. P. Lindenmaier
2000 Journal of Chromatographic Science  
A method is developed for qualitative analysis of ginkgolic acids in the leaves and fruits of Ginkgo biloba by high-performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry technique. Negative ionization mode is successful in obtaining a very abundant deprotonated molecule [M -H] -. The mass detection sensitivity is higher than ultraviolet detection but relies heavily on the concentration of acetic acid in the HPLC eluent, which consists of acetonitrile-water-acetic
more » ... d. The method is also very specific for the analysis of ginkgolic acid with no interferences from the sample matrix.
doi:10.1093/chromsci/38.4.169 pmid:10766484 fatcat:svzux5osnjcqhj5vb62xf7v7ai