The Effects of Ultraviolet Light Irradiation on Hematological and Morphological Characteristics and Potassium Level of Human Blood for Transfusion Associated Graft Versus Host Disease Prevention
Purpose-To investigate whether the whole blood and blood products UV irradiation is safe with a minimum dose which is needed for Transfusion Associated Graft Versus Host Disease (TAGVHD) prevention. The safety of 5 new inventions is also investigated. Ultraviolet irradiation has been shown to be effective for prevention. However, ionizing irradiation has not yet been replaced by ultraviolet irradiation for blood irradiation at some blood banks because there are still questions about the safety
... s about the safety of this technique. Methods/Materials-In this research, the hematological, morphological characteristics and potassium level of the irradiated blood, irradiated with an equivalent dose of 4 J/cm2 of UVC (254nm) for 3 min, which is the minimum dose shown to be effective for TAGVHD prevention according to literature available, has been studied. The data was analyzed with SPSS software. Results-The results showed that UV irradiation does not change the blood potassium level and hence does not damage the RBC membrane i.e. the correlation coefficient (cc) was 0.9879 with a p-value<0.005. Furthermore, the hematological tests showed no significant hematological change after ultraviolet exposure i.e. the cc for all of them were >0.5 with p<0.005. Moreover, the morphology of RBCs and PLTs after ultraviolet irradiation was normal (cc>0.7 with p<0.005). Conclusions-According to the results, the ultraviolet irradiation is a safe and suitable way for blood and blood component irradiation for TAGVHD prevention and other applications with an equivalent dose of up to this UV irradiation dose. Accordingly, the new proposed machines and techniques are safe to be built for TAGVHD prevention; besides a new facility in the hematology and blood banking unit may be opened for patients that have autoimmune diseases.