Lack of Enhancing Effect of Lauric Acid on the Development of Aberrant Crypt Foci in Male ICR Mice Treated with Azoxymethane and Dextran Sodium Sulfate

Shingo Miyamoto, Rikako Suzuki, Yumiko Yasui, Hiroyuki Kohno, Shigeyuki Sugie, Akira Murakami, Hajime Ohigashi, Takuji Tanaka
2007 Journal of Toxicologic Pathology  
The effect of lauric acid (LA), which is reported to induce cyclooxygenase (COX)-2 expression in macrophage cells (RAW 264.7) on the development of aberrant crypt foci (ACF), putative precursor lesions of colonic adenocarcinoma, was investigated in an inflammation-related mouse colon carcinogenesis model treated with azoxymethane (AOM) and dextran sulfate sodium (DSS). To induce ACF, male ICR mice were given a single intraperitoneal injection of AOM (10 mg/kg body weight) and then followed by
more » ... DSS in drinking water for one week, starting one week after dosing of AOM (AOM/DSS group). The AOM/DSS/LA group was fed with a diet containing 1% LA for 7 weeks, starting one week after the cessation of DSS administration. Other groups included the AOM/LA group given AOM and 1% LA diet for 9 weeks, the DSS/LA group given DSS and the diet with 1% LA, the AOM group that received AOM alone, the DSS group given DSS alone in drinking water, the LA group fed with 1% LA-containing diet alone, and the untreated group. At week 10 (end of the study), the frequency of ACF did not significantly differ between the AOM/DSS group (7.4 ± 3.0) and the AOM/DSS/LA group (8.4 ± 5.0). The value was extremely low in the AOM/LA group (1.0 ± 1.0) and in the AOM alone group (2.4 ± 2.7). No ACF developed in other groups. Our findings suggest that dietary LA did not influence the occurrence of ACF in the AOM/DSS-induced mouse colon tumorigenesis, indicating a lack of LA enhancing effects on the early phase of inflammation-related mouse colon carcinogenesis. (J Toxicol Pathol 2007; 20: 93-100)
doi:10.1293/tox.20.93 fatcat:m7oi3cnl5zgfhlz72wys75u67e