A scaled-down workflow for Illumina shotgun sequencing library preparation: lower input and improved performance at small fraction of the cost [post]

Jo Ann Tan, Alexander S Mikheyev
2016 unpublished
The high cost of library preparation remains a major obstacle to sequencing large numbers of individual genomes. Illumina's proprietary tagmentation technology allows for rapid and easy preparation of sequencing libraries, but remains prohibitively expensive for many users. Here we propose a modified version of the protocol, which uses Illumina reagents at 1/20th the scale. We show that the scaled-down protocol performs comparably to that of the manufacturer on a non-model insect genome.
more » ... sect genome. Surprisingly, the scaled-down protocol also produced 14% fewer PCR duplicates that the full-scale protocol. Since PCR duplicates effectively wasted redundant data, our protocol presented here can help save not just library preparation costs, but sequencing costs as well.
doi:10.7287/peerj.preprints.2475v1 fatcat:ppvwoow63vgufdjew7orpxrbs4