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Keratin 13 Immunostaining in Corneal Impression Cytology for the Diagnosis of Limbal Stem Cell Deficiency
2011
Investigative Ophthalmology and Visual Science
PURPOSE. The aim of this study was to develop a validated, reliable, and minimally invasive technique for diagnosing limbal stem cell deficiency (LSCD) by immunocytochemical detection of conjunctival and corneal keratins on epithelial cells collected by impression cytology (IC). METHODS. After validation of labeling techniques on a cohort of 10 healthy control patients, keratins K12, K13, and K19 were labeled on corneal IC of 10 eyes suspected of LSCD. Positive scores for the conjunctival
doi:10.1167/iovs.10-7049
pmid:22064992
fatcat:imdcxvq4bzgj3ax6erj2nu5drm