Alamethicin Channels as a Signal Transduction Element in an Immobilized Single Giant Unilamellar Vesicle

Hiroyuki Fukuda, Masaki Nitta, Misato Sakamoto, Atsushi Shoji, Masao Sugawara
2021 Sensors and materials  
Keywords: single giant unilamellar vesicle, alamethicin channel, bovine serum albumin, avidin slip A single giant unilamellar vesicle (GUV) functionalized with an anti-bovine serum albumin (BSA) antibody was immobilized on an avidin slip, and alamethicin channels were embedded as a signal transduction element for creating a channel-based molecular sensing system. The GUV sensor based on the membrane-bound anti-BSA antibody receptor exhibited alamethicin activities that reflected the binding of
more » ... ted the binding of BSA (an analyte) at the membrane/solution interface. The normalized integrated current at −60 mV was able to be used as a measure of the amount of BSA in a solution. The quantification of BSA at pg/mL level was demonstrated.
doi:10.18494/sam.2021.3067 fatcat:kvz5uikg7fgfvbk2wztckayokm