Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi

Priscilla M. Mfombep, Zachary N. Senwo, Omoanghe S. Isikhuemhen
2013 Advances in Biological Chemistry  
Beta-glucosidase is among the suite of enzymes produced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squamosus, Trametes versicolor, Lentinula edodes, and Grifola frondosa. All the WRF studied showed β-glucosidase activities. Significant variations in
more » ... variations in protein and carbohydrate contents were also recorded. Beta-glucosidase activities after 30 min of incubation ranged from 6.4 µg (T. versicolor) to 225 µg (G. frondosa). The calculated kinetic constant (K m ) ranged from 0.47 µM (A. auricular-1120) to 719 µM (L. edodes-7). The V max depending on the kinetic transformation model ranged from 0.21 µg·min −1 (T. versicolor) to 9.70 µg·min −1 (G. frondosa-28). Beta-glucosidase activities also exhibited pH optima between 3.5 and 5.0 while temperature optima were between 60˚C and 70˚C with some media exhibiting a secondary temperature peak at 90˚C attributable to the presence of thermostable isoenzyme. WRF if appropriately screened and purified can be harnessed to potentially improve the bioconversion of cellulose to glucose and also facilitate efficient plant biomass biodegradation and production of useful plant bio-products.
doi:10.4236/abc.2013.32025 fatcat:dagtk6qdgzb67jt37642lwa2de