A 1.5-Mb-resolution radiation hybrid map of the cat genome and comparative analysis with the canine and human genomes

William J. Murphy, Brian Davis, Victor A. David, Richa Agarwala, Alejandro A. Schäffer, Alison J. Pearks Wilkerson, Beena Neelam, Stephen J. O'Brien, Marilyn Menotti-Raymond
2007 Genomics  
We report the construction of a 1.5-Mb-resolution radiation hybrid map of the domestic cat genome. This new map includes novel microsatellite loci and markers derived from the 2X genome sequence that target previous gaps in the feline-human comparative map. Ninety-six percent of the 1793 cat markers we mapped have identifiable orthologues in the canine and human genome sequences. The updated autosomal and X-chromosome comparative maps identify 152 cat-human and 134 cat-dog homologous synteny
more » ... cks. Comparative analysis shows the marked change in chromosomal evolution in the canid lineage relative to the felid lineage since divergence from their carnivoran ancestor. The canid lineage has a 30-fold difference in the number of interchromosomal rearrangements relative to felids, while the felid lineage has primarily undergone intrachromosomal rearrangements. We have also refined the pseudoautosomal region and boundary in the cat and show that it is markedly longer than those of human or mouse. This improved RH comparative map provides a useful tool to facilitate positional cloning studies in the feline model. Genomics 89 (2007) 189 -196 www.elsevier.com/locate/ygeno ⁎ Corresponding authors. W.J. Murphy is to be contacted at fax: +1 979 845 9972. M. Menotti-Raymond,
doi:10.1016/j.ygeno.2006.08.007 pmid:16997530 pmcid:PMC3760348 fatcat:67ibvq6kcrh7jfv2wuw3igeabi