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A rapid resolution liquid chromatographic (RRLC) method for the determination of eight polyphenols in tobacco was developed. Polyphenols were extracted from tobacco samples by methanol/ water in an ultrasonic bath, then subjected to clean up by solid phase extraction. The separation was performed on a 50 × 4.6 mm, 1.8 μm ZORBAX Eclipse XDB-C18 column. Compared with conventional HPLC method, the analysis time for eight polyphenols by RRLC method was reduced from 20 to 5 min without sacrificingdoi:10.4236/ajac.2011.28107 fatcat:vkr52palxbd4nngayy3a4qkv5i