New Generation Ecotox - Biosystem of water monitoring through analysis of real-time image

Hueih- Min Chen
2011 Journal of Analytical & Bioanalytical Techniques  
H igh-molecular (>1000 kDa) preparations from the crude polysaccharides of Symphytum asperum and S.caucasicum roots were isolated by ultrafi ltration on membrane fi lters with cut-off values of 1000 kDa and the elucidation of their principal structural units was carried out. According to IR, 13 C NMR, 1 H NMR, 2D heteronuclear 1 H/ 13 C HSQC spectral data the main structural element of these preparations was found to be a regularly substituted polyoxyethylene, namely
more » ... -1-carboxy-2-(3,4dihydroxyphenyl)ethylene] or poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA). Such phenolic biopolymer to our knowledge has not been known and has been identifi ed for the fi rst time. Th is compound represents a new class of natural polyethers with a residue of 3-(3,4-dihydroxyphenyl)glyceric acid as the repeating unit. PDPGA possesses anticomplementary, antioxidant and anti-infl ammatory activities. We examined the effi cacy of PDPGA of S.asperum (PDGPA-SA) and S.caucasicum (PDGPA-SC) in androgen-dependent (LNCaP) and -independent (22Rv1 and PC3) human prostate cancer (PCA) cells. PDPGA-SA treatment (100 mcg/ml for 48h) decreases the live cell number by 65, 64 and 35% and increases the cell death by 16, 8 and 12 folds in LNCaP, 22Rv1 and PC3 cells, respectively. Similarly, PDPGA-SC treatment (100 mcg/ml for 48h) decreased the live cell number by 87, 25 and 33% and increased the cell death by 19, 10 and 9 folds in LNCaP, 22Rv1 and PC3 cells, respectively. Overall, present study identifi ed the strong effi cacy of these novel phytochemicals against PCA cells, and suggested that their pre-clinical effi cacy studies in PCA models are needed in future to move them forward for possible translational potential in PCA patients. Biography and
doi:10.4172/2155-9872.s1.02 fatcat:5vdqlkbvkba3bkp3e3lkbteyxq