Developing an anion host for lipid A binding and antibacterial activity

Luke C. Henderson, Jian Li, Roger L. Nation, Tony Velkov, Frederick M. Pfeffer
2010 Chemical Communications  
General Experimental All 1 H and 13 C NMR spectra were recorded on a Jeol JNM-EX 270 MHz or Eclispe 400 MHz FT-NMR spectrometer as indicated. Samples were dissolved in deuterated chloroform (CDCl 3 ), with the residual solvent peak used as an internal reference (7.26 ( 1 H NMR) and 77.10 ( 13 C NMR)). Proton spectra are reported as follows: chemical shift δ (ppm), (integral, multiplicity (s=singlet, br s = broad singlet, d = doublet, dd = doublet of doublets, t = triplet, q = quartet, m =
more » ... let), coupling constant J (Hz). Carbon spectra are reported as chemical shift δ (ppm). Fourier transform infrared (FT-IR) spectra were recorded on an Excalibur Series Biorad FT-IR spectrometer. High Resolution Mass Spectra (HRMS) were recorded on a 6210 MSD TOF mass spectrometer (Agilent Technologies, Australia) with the following conditions: drying gas nitrogen (7.0 L/min, 325 ºC); nebuliser gas nitrogen (15 psi); capillary voltage 3.0 kV; vaporiser temperature 29 ºC; and cone voltage 40 V. Methanol was used as the mobile phase. Samples were dissolved in methanol, with less than 1 mg of sample per mL of solvent. Thin Layer Chromatography (TLC) was performed using aluminium-
doi:10.1039/b925135a pmid:20424772 fatcat:mu74l2fh7vch5eyy5refknpbou