Structural and biochemical studies of Sporosarcina pasteurii UreE: a nickel-chaperone involved in the urease activation process Coordinatore Dottorato Relatore

Presentata, Dott Ssa, Anna Merloni, Vincenzo Scarlato, Stefano Ciurli
unpublished
Esame finale anno 2014 7.1.2. SpUreE-SpUreG by NMR 102 -Assignment of the NMR spectra of SpUreE and intrinsic exchange rates -Secondary structure propensity of SpUreE from NMR chemical shifts -NMR studies of SpUreE dynamics -CSP analysis of SpUreE-SpUreG interaction 7.2. Characterization of metal binding properties of SpUreE 115 7.2.1. Nickel binding by ITC 116 7.2.2. Zinc binding by ITC 118 7.2.3. Metal binding by X-ray crystallography 120 7.2.4. Ni(II) and Zn(II) competition for SpUreE 123 8.
more » ... Conclusions 129 9. Supplementary information 135 Bibliography 141 Acnowledgements 149 6 7
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