A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
The Catalytic Domain of Protein Kinase C-δ in Reciprocal δ and ϵ Chimeras Mediates Phorbol Ester-induced Macrophage Differentiation of Mouse Promyelocytes
Journal of Biological Chemistry
The overexpression of protein kinase C-␦ (PKC-␦), but not PKC-⑀, enables the mouse myeloid cell line 32D to differentiate into macrophages when treated with phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA). To determine the domain of PKC-␦ that is responsible for this isotype-specific function, cDNAs that encode reciprocal chimeras of PKC-␦ and -⑀ (PKC-␦⑀ and PKC-⑀␦) were constructed by exchanging regulatory and kinase domains using polymerase chain reaction technology. Bothdoi:10.1074/jbc.272.1.76 pmid:8995230 fatcat:fzbbpnr5tnar3fmlygq4oril7q