Solan (HP)-173230, India 2 Nivedita Sharma (PhD)

Nisha Sharma, Nivedita Sharma
2017 WJPRT   unpublished
During lignocellulosic hydrolysis, a large variety of polyaromatic and phenolic compounds are released due to lignin and sugar degradation. They are toxic to fermenting microorganisms even more than furan limiting the microbial growth. They cause a partition and disturb the membranes in turn affecting their ability to serve as selective barriers and enzyme matrices affecting the cell growth and sugar assimilation. In the present study, bioethanol production from alkaline hydrogen peroxide
more » ... ogen peroxide pretreated Populus deltoides wood under simultaneous saccharification and fermentation (SHF) was done. During the bioethanol production, there is generation of inhibitors like ferulic acid, levulinic acid, vanillin, acetic acid, 5-hydroxy methyl furfurals (5-HMF) and furfurals. 5-Hydroxy methyl furfural is one of the most important inhibitor generated the degradation of sugars resulting in the toxicity of fermenting liquor and thus limiting the fermentation. 5-Hydroxymethyl furfural (5-HMF) is a well known heterocyclic Maillard reaction product has often been studied for its potential toxic, mutagenic and carcinogenic effects. High performance liquid chromatography is a powerful and widely used technique for the separation of biomolecules, both large and small. Its primary advantages over other separation modes are high efficiency and the ability to distinguish between compounds that are chemically very similar. So here we used HPLC technique to detect the quantity of 5-HMF during steps involved in bioethanol fermentation.