Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal pathogenesis

A P Vicentini, J L Gesztesi, M F Franco, W de Souza, J Z de Moraes, L R Travassos, J D Lopes
1994 Infection and Immunity  
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more » ... -Universidade Estadual Paulista Extracellular matrix protein laminin binds specifically to yeast forms of Paracoccidioides brasiliensis and enhances adhesion of the fungus to the surface of epithelial Madin-Darby canine kidney cells in vitro. Immunoblotting of fungal extracts showed that the gp43 glycoprotein is responsible for adhesion. This was confirmed by binding assays using purified gp43, with a Kd of 3.7 nM. The coating of P. brasiliensis yeast forms with laminin before injection into hamster testicles enhanced the fungus virulence, resulting in a faster and more severe granulomatous disease. These results indicate that interaction of fungi with extracellular matrix elements may constitute a basis for the evolution of fungal infection toward regional spreading and dissemination.
doi:10.1128/iai.62.4.1465-1469.1994 fatcat:zzn7mqfbvrhodesek4xzgbvxxy