Isolation and identification of adenosine 5'-phosphosulphate(ASP) as a reaction product of ATP-sulphurylase

Nobuhiro Kanno, Minoru Sato, Yoshikazu Sato
1987 Nippon Suisan Gakkaishi  
APS formation catalyzed by purified ATP-sulphurylase of Porphyra yezoensis was demonstrated. APS accumulated in the incubated enzyme mixture was separated on a DEAE-Toyo pearl 650M column, and identified by anion-exchange HPLC and TLC. UV absorption spectra, acid-lability, and equimolar contents in adenine, ribose, phosphate and sulphate also substantiated the identity. The low accumulation of APS suggested the presence of some difficulties in the APS synthesizing reaction. ATP-sulphurylase (EC
more » ... 2. 7. 7. 4.) catalyzes the first step in the sulphate activating reaction: SO42-+ATP ?? APS+PPi
doi:10.2331/suisan.53.1867 fatcat:cpd3sgqb2bdd3or7tpfelgixnu