A Fine Physical Map of Arabidopsis thaliana Chromosome 5: Construction of a Sequence-ready Contig Map

H. Kotani
1997 DNA Research  
A fine physical map of Arabidopsis thaliana chromosome 5 was constructed by ordering the clones from YAC, PI, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of PI, TAC and BAC clones. The entire genome of chromosome 5, except for the centromeric and telomeric regions, was covered by two
more » ... covered by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region. The minimum tiling path of the chromosome was constituted by a total of 430 PI, TAC and BAC clones. The map information is available at the Web site http://www.kazusa.or.jp/arabi/.
doi:10.1093/dnares/4.6.371 pmid:9501992 fatcat:eocfszviizcungwbqhwoli4pk4