Regulation of miR-375 and Sonic hedgehog on vascular endothelial growth factor in preeclampsia rats and its effect on trophoblast cells

Yanfang Ren, Yifang Xu, Yuanyuan Wang, Yongjie Jiang, Jianguo Wen
2020 Bioscience Reports  
Purpose: To study the mechanism of vascular endothelial growth factor (VEGF) regulated by miR-375 and Sonic hedgehog (SHH) signaling pathways on the characteristics of trophoblast cells (TCs) in preeclampsia (PE) rats. Methods: Female (100) Wistar rats in rut were mated with males (50) at a ratio of 2:1, and 60 pregnant rats were assigned to a normal group (Nor, n=10) and a model group (Mod, n=50) in a random manner. Rat TCs of placental villus were isolated and were divided into 8 groups. The
more » ... elated indicators in each group were detected, respectively. Results: In contrast to the NC group, miR-375 expression greatly elevated in the miR-375 mimic group (mimic group); in the mimic and cyclopamine groups, VEGF, proliferating cell nuclear antigen(PCNA, N- cadherin and Bcl-2 expression, cell proliferation, S phase cells, and migration and invasion ability were significantly decreased and E-cadherin and Bax expression, G1 phase cells and apoptosis rate were increased significantly, but in Over expressing-VEGF(ov-VEGF) group, the above indicators presented contrary results (all P< 0.05). In contrast to the mimic group, the expression of SHH, VEGF, PCNA, N- cadherin and Bcl-2, cell proliferation, S phase cells and the invasion and migration activity in the miR-375 mimic + oe-VEGF group were evidently increased. Conclusions: Over-expressing miR-375 can inhibit the expression of VEGF, thus slow down the invasion and proliferation of TCS in PE rats, which is realized by regulating SHH signal pathway.
doi:10.1042/bsr20200613 fatcat:5fhzbtb7jbcodovtjfhec3fak4