Screening of Class 1 and Class 2 Integrons in Clinical Isolates of Pseudomonas aeruginosa Collected from Seven Hospitals in Turkey: A Multicenter Study

Aysegul Copur Cicek, Aysegul Saral, Azer Ozad Duzgun, Zeynep Cizmeci, Tuba Kayman, Pervin Ozlem Balci, Tuba Dal, Mehmet Firat, Yelda Yazici, Metin Sancaktar, Osman Birol Ozgumus, Cemal Sandalli
2013 Open Journal of Medical Microbiology  
Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integrons and antibiotic susceptibility of clinical isolates of P. aeruginosa. Two hundred and five P. aeruginosa strains were collected from the seven general state hospitals in Turkey. They were characterized by antimicrobial susceptibility testing, screened for class 1 and class 2
more » ... 1 and class 2 integrons, and evaluated for the association between antibiotic resistance phenotypes and the presence of integrons. intI gene was amplified in 10 isolates (4.87%) by PCR and in seven isolates of them (70%) were found different gene cassettes. The aadA gene integrated into the class 1 integrons was most frequently found and it was followed by aac genes and bla OXA family genes. Sequence analysis of variable regions of the class 1 integrons showed five gene cassette arrays;
doi:10.4236/ojmm.2013.34034 fatcat:2t4glaueungazhedxs4pobvzbu