CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle

Xuan Zhou, Defa Li, Jingdong Yin, Jianjun Ni, Bing Dong, Jinxiao Zhang, Min Du
2007 Journal of Lipid Research  
Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood. The objective of this study was to investigate the different effects of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular cells obtained from neonatal
more » ... d from neonatal pigs. As shown here, trans -10, cis -12 CLA decreased the expression of adipocytespecific genes as well as adipose precursor cell numbers and the accumulation of lipid in cultured SC adipose tissue stromal vascular cells. However, the cis -9, trans -11 CLA did not alter adipogenesis in SC cultures. On the other hand, both CLA isomers increased the expression of adipocytespecific genes in IM cultures, together with the increasing accumulation of lipid and Oil Red O-stained cells. Collectively, these data show that CLA decreases SC adipose tissue but increases IM adipose tissue by different regulation of adipocyte-specific gene expression. These results suggest that adipogenesis in IM adipocytes differs from that in SC adipocytes
doi:10.1194/jlr.m600525-jlr200 pmid:17496270 fatcat:oxipftoq2fbipm26td3nuwbave