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To explain the observation in field experiments that tobacco variety CB-1 was more nitrogen (N)-efficient than K326, the influence of two N levels on growth, N uptake and N flow within plants of the two tobacco varieties was studied. Xylem sap from the upper and lower leaves of both tobacco varieties cultured in quartz sand was collected by application of pressure to the root system. CB-1 took up more N with smaller roots at both high (HN, 10 mmol L −1 ) and low (LN, 1 mmol L −1 ) N levels, anddoi:10.1016/j.cj.2014.08.008 fatcat:teiopuz2rzftrlbguz6f47bjvq