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Raman microscopy enables the delimitation of the dentin-enamel junction (DEJ) in human and animal teeth, by using, e.g., the 1 oscillations of PO43- (960 cm-1) for the boundary with the enamel and of stretching modes for C-H2 (~2935 cm-1) for the boundary with the dentin and it can be strictly compared with the optical image of DEJ. In this way, one can observe the distribution of some elements of collagen fibrils crossing DEJ, by using the oscillations for proline ((C-C) of ring at 855 anddoaj:ebd294e31e54453690a94cb61a97278b fatcat:y2wboccukvhyjpafviatxm7ylq