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protein epitopes, the sequence alignment of p26 protein was performed for four major monophyletic groups isolated in North American, China, southern Japan and Ireland respectively. The alignment analysis revealed that the defined linear epitopes were not highly conserved. The corresponding mutants were constructed and expressed in HEK293T cells. The cross-reactivity between MAbs and their respective variant epitopes was analyzed with western blotting and AC-ELISA. The results showed that 9H8doi:10.1016/j.jevs.2016.02.076 fatcat:w7yeqbhcrzagnltvixhbkkds7q