Seropositivity of Equine infectious anemia by 2005 to 2014 in provinces of north-west of Argentina

C.A. Storani, R.A. Jacobo, M.F. Cipolini, D.E. Martinez, A.G. Espasandín, V. Paine, J.A. Godoy
2016 Journal of Equine Veterinary Science  
protein epitopes, the sequence alignment of p26 protein was performed for four major monophyletic groups isolated in North American, China, southern Japan and Ireland respectively. The alignment analysis revealed that the defined linear epitopes were not highly conserved. The corresponding mutants were constructed and expressed in HEK293T cells. The cross-reactivity between MAbs and their respective variant epitopes was analyzed with western blotting and AC-ELISA. The results showed that 9H8
more » ... showed that 9H8 could only recognize EIAV strains isolated from China and Japan, while 1G11 could react with all the four EIAV strains. This result indicated that 1G11 have a broadly recognized capacity to p26 epitope from different EIAV strains and could serve as a useful tool for the development of new methods on the anti-EIAV antibody determination.
doi:10.1016/j.jevs.2016.02.076 fatcat:w7yeqbhcrzagnltvixhbkkds7q