Comparative analyses of fungal biota carried by the pine shoot beetle (Tomicus piniperdaL.) in northern and southern Finland

Ximena Silva, Eeva Terhonen, Hui Sun, Risto Kasanen, Kari Heliövaara, Risto Jalkanen, Fred O. Asiegbu
2015 Scandinavian Journal of Forest Research  
The pine shoot beetle, Tomicus piniperda L., is a secondary colonizer of pines and other conifers. Although it is necessary to understand interactions of this insect with other organisms, few studies have focused on its fungal associates. This study focused on the effect of geographical distance on the occurrence of fungi carried by the beetle. Adult beetles were collected from Scots pine in northern (Rovaniemi) and southern (Hyytiälä) Finland. Both epi-and endo-mycota were isolated, identified
more » ... solated, identified using a combination of morphological and molecular data. The results revealed a great diversity of fungal taxa isolated from T. piniperda, with a total of 3073 isolates representing 26 taxanomic units. The most frequently isolated fungi in the bark beetles from northern Finland were Beauveria bassiana (Bals.-Criv.) Vuill., Kuraishia sp. whereas Penicillium brevicompactum Dierckx and Mortierella sp. were mostly observed in the insects from southern Finland. Ophiostoma canum (Münch) Syd. & P. Syd. and O. minus (Hedgc.) Syd. & P. Syd. were also isolated from the beetles from both northern and southern Finland. The number of epi-and endo-mycota isolates per insect was 2.83 and 2.38 in the north, and 4.1 and 3.5 in the south, respectively. Significant differences were found between southern and northern Finland in fungal taxa isolated from the beetles. The highest richness and diversity of the fungal taxa was observed in the south. However, the overall fungal diversity index analysis revealed that the mycobiota was under-sampled which suggests that a proportion of the fungal species may have remained undetected.
doi:10.1080/02827581.2015.1031824 fatcat:xlwtjp74ibb2fo5np65slyc3pi