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Bioluminescent Monitoring of ATP Release from Human Red Blood Cells Treated with Nonionic Detergent

Tamás Köszegi, Miklós Kellermayer, Ferenc Kövecs, Kázmér Jobst
1988 Clinical Chemistry and Laboratory Medicine  

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The adaptation of the firefly luciferin-luciferase system for the detection of ATP in the presence of Triton X 100 or Brij 58 nonionic detergents is described. The method is sensitive, easy to perform and is suitable for the continuous monitoring of the mobilization rate of ATP from Triton X 100-or Brij 58-treated human erythrocytes. A very rapid ATP release was observed after Triton X 100 treatment of the red blood cells while the presence of Brij 58 caused a retarded mobilization of the
more » ... ellular ATP. The technique described seems to be applicable to all kinds of cells.
doi:10.1515/cclm.1988.26.10.599 fatcat:gjxs5vhudvc6vex5ql7iyudd6q
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Tamás Köszegi, Miklós Kellermayer, Ferenc Kövecs, Kázmér Jobst. "Bioluminescent Monitoring of ATP Release from Human Red Blood Cells Treated with Nonionic Detergent." Clinical Chemistry and Laboratory Medicine 26.10 (1988)