Bioluminescent Monitoring of ATP Release from Human Red Blood Cells Treated with Nonionic Detergent

Tamás Köszegi, Miklós Kellermayer, Ferenc Kövecs, Kázmér Jobst
1988 Clinical Chemistry and Laboratory Medicine  
The adaptation of the firefly luciferin-luciferase system for the detection of ATP in the presence of Triton X 100 or Brij 58 nonionic detergents is described. The method is sensitive, easy to perform and is suitable for the continuous monitoring of the mobilization rate of ATP from Triton X 100-or Brij 58-treated human erythrocytes. A very rapid ATP release was observed after Triton X 100 treatment of the red blood cells while the presence of Brij 58 caused a retarded mobilization of the
more » ... ellular ATP. The technique described seems to be applicable to all kinds of cells.
doi:10.1515/cclm.1988.26.10.599 fatcat:gjxs5vhudvc6vex5ql7iyudd6q